BCR antibody (Tyr177)

Catalog No. ABIN197420

The Rabbit Polyclonal anti-BCR antibody is suitable to detect BCR in samples from Human and Mouse. It has been validated for WB and IF.

Quick Overview for BCR antibody (Tyr177) (ABIN197420)

Target: BCR (Breakpoint Cluster Region (BCR))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCR antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-BCR Antibody

Binding Specificity: Tyr177

Specificity: This antibody AP02709PU detects endogenous levels of total Bcr protein.

Purification: Immunoaffinity Chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Bcr around the phosphorylation site of tyrosine 177 (P-F-Yp-V-N).

Application Details

Application Notes: Western blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for BCR

Target: BCR (Breakpoint Cluster Region (BCR))

Alternative Name: BCR

Background: The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42. Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling.Synonyms: BCR1, Breakpoint cluster region protein, D22S11, NY-REN-26, Renal carcinoma antigen NY-REN-26

Gene ID: 613

NCBI Accession: NP_004318

UniProt: P11274

Pathways: Regulation of Leukocyte Mediated Immunity, Platelet-derived growth Factor Receptor Signaling