Fc gamma RII (CD32) antibody

Catalog No. ABIN1981874

The Mouse Monoclonal anti- antibody has been validated for FACS and MS. It is suitable to detect in samples from Human. There are 4+ publications available.

Quick Overview for Fc gamma RII (CD32) antibody (ABIN1981874)

Target: Fc gamma RII (CD32)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Mass Spectrometry (MS)

Clone: 3D3

Product Details

Purpose: Anti-Hu CD32 Purified

Specificity: The mouse monoclonal antibody 3D3 recognizes an extracellular epitope of CD32, a 40 kDa polymorphic transmembrane glycoprotein serving as the low affinity receptor for aggregated IgG. This antibody recognizes CD32 isoforms on B cells of all donors, but on platelets, monocytes, and granulocytes of only some donors (131R variant, but not 131H variant).

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: purified glycosylated recombinant human FcgammaRIIa2

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 2-6 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Do not use after expiration date stamped on vial label.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References

Os, Bürgler, Ribes, Funderud, Wang, Thompson, Tjønnfjord, Bogen, Munthe: "Chronic lymphocytic leukemia cells are activated and proliferate in response to specific T helper cells." in: Cell reports, Vol. 4, Issue 3, pp. 566-77, (2013) (PubMed).

Dutertre, Bonnin-Gélizé, Pulford, Bourel, Fridman, Teillaud: "A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function." in: Journal of leukocyte biology, Vol. 84, Issue 6, pp. 1511-20, (2008) (PubMed).

Boruchov, Heller, Veri, Bonvini, Ravetch, Young: "Activating and inhibitory IgG Fc receptors on human DCs mediate opposing functions." in: The Journal of clinical investigation, Vol. 115, Issue 10, pp. 2914-23, (2005) (PubMed).

Vely, Gruel, Moncuit, Cochet, Rouard, Dare, Galon, Sautes, Fridman, Teillaud: "A new set of monoclonal antibodies against human Fc gamma RII (CD32) and Fc gamma RIII (CD16): characterization and use in various assays." in: Hybridoma, Vol. 16, Issue 6, pp. 519-28, (1998) (PubMed).

Target Details

Target: Fc gamma RII (CD32)

Alternative Name: CD32

Background: Fc fragment of IgG receptor IIa,CD32 (FcgammaRII) is a low affinity receptor for aggregated IgG. It is strongly expressed on monocytes, granulocytes, myeloid and myeloblastic cell lines, and weakly on B cells, CD34+ bone marrow cells, and resting and activated platelets. After binding its ligand, CD32 induces IgG-mediated phagocytosis and oxidative burst in monocytes and neutrophils, whereas in B cells it mediates a negative signal. This polymorphic transmembrane glycoprotein is expressed not only in the activating (CD32a) and inhibitory isoform (CD32b), but also in individual variants with differing avidities for IgG subtypes (e.g. the CD32a131R and CD32a131H allotypes).,FCG2, FCGR2A, IGFR2

Gene ID: 2212

UniProt: P12318