VASP antibody (pSer239)

Catalog No. ABIN198238

This Mouse Monoclonal antibody specifically detects VASP in WB, ELISA, IP, ICC and FACS. It exhibits reactivity toward Human, Mouse, Rat, Monkey, Dog, Cow and Horse.

Quick Overview for VASP antibody (pSer239) (ABIN198238)

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Reactivity: Human, Mouse, Rat, Monkey, Dog, Cow, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This VASP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 16C2

Product Details anti-VASP Antibody

Binding Specificity: AA 236-242, pSer239

Specificity: Recognizes human phosphorylated VASP, Mr 50kD. Species cross-reactivity: mouse.

Purification: Gel filtration

Immunogen: KLH-conjugated, synthetic phosphopeptide corresponding to amino acids 236-242 of human phospho-VASP (RKV[pS]KQE).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.05 μg/mL), Flo (2.4 μg/mL), ICC, IP, WB

Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry, FACS and Immunoprecipitation. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: An independent laboratory has reported that 3 μg/mL antibody showed positive immunostaining in SNP or 8pCPT-cGMP-treated human platelets, fixed with 4 % paraformaldehyde and permeabilized with 0.2 % Triton-X. FACS: 2.4 μg/mL using fixed and permeabilized human platelets, as reported by an independent laboratory. Immunoprecipitation: Immunoprecipitates phosphorylated VASP from vanadate-treated A431 cells, as reported by an independent laboratory. ELISA: 0.05 μg/mL, as reported by an independent laboratory.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 1 mL ddH2O

Concentration: Lot specific

Buffer: Supplied as a lyophilized powder from PBS, 0.09 % sodium azide, PEG, sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for VASP

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Alternative Name: VASP

Background: Name/Gene ID: VASP
Family: Ena-VASP

Synonyms: VASP

Gene ID: 7408

UniProt: P50552

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation