Cx40/GJA5 antibody (C-Term)

Catalog No. ABIN199015

This Rabbit Polyclonal antibody specifically detects Cx40/GJA5 in WB, IHC, IF, IP and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for Cx40/GJA5 antibody (C-Term) (ABIN199015)

Target: Cx40/GJA5 (GJA5) (Gap Junction Protein, alpha 5, 40kDa (GJA5))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cx40/GJA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Cx40/GJA5 Antibody

Binding Specificity: C-Term

Specificity: Reacts with the mouse Connexin 40 protein. On Western Blots, it identifies the target band at ~40kD. Reactivity has been confirmed with mouse heart homogenates by Western Blot, mouse heart and lung homogenates by immunoprecipitation, and mouse heart tissue by immunohistochemistry and immunofluorescence. Species cross-reactivity: Cross-reactivity with rat and human CX40 proteins is expected based on amino acid sequence homology.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the C-terminal region of the mouse and rat Connexin 40 (CX40) proteins. The human sequence at this location differs by only one amino acid.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IF (1 - 3 μg/mL), IHC, IHC-Fr, IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Western Blot: 1-3 μg/mL. Immunofluorescence: 1-3 μg/mL. Immunoprecipitation: 7 μg/reaction. Immunohistochemistry (Frozen sections): 1-3 μg/mL, For IHC, mice were perfused transcardially with 3 mL pre-fixative (50 mM phosphate buffer, 0.1 % sodium nitrite, 1 unit/mL heparin),. followed by 40 mL fixative (0.16M sodium phosphate buffer, 0.2 % picric acid, and 1-, 2-, or 4 % paraformaldehyde), and perfusion with 10 mL sucrose wash (10 % sucrose in 25 mM sodium phosphate buffer,  pH 7.4). Tissues were then stored in cryoprotectant (10 % sucrose, 25 mM phosphate buffer,  pH 7.4, 0.04 % sodium azide) for a minimum of 24 hours prior to sectioning.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Cx40/GJA5

Target: Cx40/GJA5 (GJA5) (Gap Junction Protein, alpha 5, 40kDa (GJA5))

Alternative Name: GJA5 / CX40 / Connexin 40

Background: Name/Gene ID: GJA5
Subfamily: Connexin
Family: Ion Channel

Synonyms: GJA5, Connexin 40, Connexin-40, Gap junction alpha-5 protein, ATFB11, CX40, Gap junction protein alpha 5

Gene ID: 2702

UniProt: P36382

Pathways: Cell-Cell Junction Organization