GJB1 antibody (C-Term)

Catalog No. ABIN200657

The Mouse Monoclonal anti-GJB1 antibody has been validated for WB, IHC and IF. It is suitable to detect GJB1 in samples from Human, Rat and Mouse.

Quick Overview for GJB1 antibody (C-Term) (ABIN200657)

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This GJB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-GJB1 Antibody

Binding Specificity: C-Term

Specificity: Reacts with both the monomer and dimer forms of Connexin 32 (molecular weights of ~32kD and 51kD). Reactivity is confirmed with brain and liver from rat and mouse. Based on sequence homology, this antibody is expected to react with human Connexin 32.

Purification: Protein A purified

Immunogen: Synthetic peptide derived from the C-terminus of mouse.

Type of Immunogen: Synthetic peptide

Isotype: IgG2a

Application Details

Application Notes: Approved: IF (10 μg/mL), IHC (10 μg/mL), WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry. Immunofluorescence: 10 μg/mL. Western Blot: 1-3 μg/mL. Immunohistochemistry: 10 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for GJB1

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Alternative Name: GJB1 / CX32 / Connexin 32

Background: Name/Gene ID: GJB1
Subfamily: Connexin
Family: Ion Channel

Synonyms: GJB1, Connexin 32, CMTX1, Connexin-32, CX32, Gap junction beta-1 protein, Gap junction protein beta-1, CMTX

Gene ID: 2705

UniProt: P08034

Pathways: Cell-Cell Junction Organization