GAD antibody

Catalog No. ABIN200986

This Mouse Monoclonal antibody specifically detects GAD in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GAD antibody (ABIN200986)

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GAD Antibody

Specificity: Recognizes rat GAD67. No detectable cross-reactivity with GAD65 by Western Blot on rat brain lysate. Species cross-reactivity: human and mouse.

Purification: Protein A purified

Immunogen: Recombinant rat GAD67 protein.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: IHC, IHC-P, WB (1:5000 - 1:10000)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:5000-1:10000, Using ECL on rat and mouse brain lysate. Immunohistochemistry (Paraffin): 1:500, No epitope retrieval necessary. Positive control: SKNSH cell lysate (human neuroblastoma), mouse cerebral cortex.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M PBS,  pH 7.6, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for GAD

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Alternative Name: GAD67 / GAD

Background: Name/Gene ID: GAD1

Synonyms: GAD1, CPSQ1, GAD-67, Glutamate decarboxylase 1, GAD67, GAD, SCP

Gene ID: 2571

UniProt: Q99259