DYKDDDDK Tag antibody (iFluor 488)

Catalog No. ABIN2017574

Product Details anti-DYKDDDDK Tag Antibody (iFluor 488)

Target: DYKDDDDK Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This DYKDDDDK Tag antibody is conjugated to iFluor 488

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Brand: THE™

Sequence: DYKDDDDK

Characteristics: THETM DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse is THETM DYKDDDDK Tag Antibody (A00187) conjugated with iFluor 488 under optimal conditions with an F/P ratio of 4-8. It is suitable for detecting the expression level of DYKDDDDK-tagged proteins. iFluor 488 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 488.

Purification: Protein A affinity column

Immunogen: A synthetic peptide (DYKDDDDK) coupled to KLH

Clone: 5A8E5

Isotype: IgG2b kappa

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescent staining: 1-4 μg/mL
Flow cytometry: 1-4 μg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.

Expiry Date: 12 months

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Abstract: DYKDDDDK Tag Products

Target Type: Tag

Background: The Flag tag is a polypeptide of with the amino acid sequence DYKDDDDK amino acid sequence that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of the protein to facilitate detection and purification. An anti-Flag tag antibody is a useful tool for the analysis of Flag-tagged proteins.