ESAM antibody

Catalog No. ABIN204196

This Mouse Monoclonal antibody specifically detects ESAM in WB, ELISA, ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for ESAM antibody (ABIN204196)

Target: ESAM (Endothelial Cell Adhesion Molecule (ESAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ESAM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-ESAM Antibody

Specificity: Selected for its ability to detect human ESAM in direct ELISAs and Western Blots. In these applications, this antibody shows no cross-reactivity with rmESAM.

Purification: Protein G purified

Immunogen: NS0-derived rhESAM.

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA, Flo, ICC, WB

Usage: Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect human ESAM. Using a colorimetric detection system, the detection limit for rhESAM is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was validated for flow cytometry using HUVECs. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. HUVECs were stained with anti-ESAM or isotype control antibody, followed by PE-conjugated anti-mouse antibody. Immunocytochemistry: This antibody was used at a concentration of 10 μg/mL to detect ESAM in HUVECs. Cells were fixed with PBS containing 4 % paraformaldehyde and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA. After blocking, cells were incubated with diluted primary antibody followed by NL557-coupled anti-mouse IgG in the dark. Between each step, cells were washed with PBS containing BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human ESAM. The detection limit for rhESAM is approximately 1 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for ESAM

Target: ESAM (Endothelial Cell Adhesion Molecule (ESAM))

Alternative Name: ESAM

Background: Name/Gene ID: ESAM

Synonyms: ESAM, LP4791 protein, W117m

Gene ID: 90952

UniProt: Q96AP7