PVRL4 antibody

Catalog No. ABIN204425

The Rat Monoclonal anti-PVRL4 antibody has been validated for WB and ELISA. It is suitable to detect PVRL4 in samples from Mouse.

Quick Overview for PVRL4 antibody (ABIN204425)

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This PVRL4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PVRL4 Antibody

Specificity: Selected for its ability to detect mouse Nectin-4 in direct ELISAs and Western Blots. In these formats, this antibody shows 100 % cross-reactivity with rhNectin-4, 10 % cross-reactivity with rmNectin-3, and no cross-reactivity with rmNectin-1 or rmNectin-2.

Purification: Protein G purified

Immunogen: NS0-derived rmNectin-4.

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, WB

Usage: Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect mouse Nectin-4. Using a colorimetric detection system, the detection limit for rmNectin-4 is approximately 20 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse Nectin-4. The detection limit for rmNectin-4 is approximately 10 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for PVRL4

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Alternative Name: PVRL4 / Nectin 4

Background: Name/Gene ID: PVRL4

Synonyms: PVRL4, EDSS1, Nectin 4, Ig superfamily receptor LNIR, Poliovirus receptor-like 4, LNIR, Nectin-4, Poliovirus receptor-related 4

Gene ID: 81607

UniProt: Q96NY8

Pathways: Cell-Cell Junction Organization