Occludin antibody (C-Term) (HRP)

Catalog No. ABIN204501

This anti-Occludin antibody is a Mouse Monoclonal antibody detecting Occludin in WB, ELISA and IF. Suitable for Human, Mouse, Rat and Dog.

Quick Overview for Occludin antibody (C-Term) (HRP) (ABIN204501)

Target: Occludin (OCLN)

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Occludin antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: OC-3F10

Product Details anti-Occludin Antibody (HRP)

Binding Specificity: C-Term

Specificity: Recognizes Occludin Protein. Species cross-reactivity: Human, mouse, rat and canine.

Purification: Protein A purified

Immunogen: GST fusion protein consisting of the C-terminal region (~150aa) of human occludin.

Type of Immunogen: Fusion protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF, WB (0.1 - 1 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunofluorescence. ELISA: 0.1-1 μg/mL. Western Blot: 0.1-1 μg/mL. Positive control: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), Caco-2 cells (human colon adenocarcinoma) and rat liver. O5200 does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 1 % BSA, 0.1 % Proclin, 40 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Occludin

Target: Occludin (OCLN)

Alternative Name: OCLN / Occludin

Background: Name/Gene ID: OCLN

Synonyms: OCLN, Occludin, BLCPMG

Gene ID: 100506658

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C