TCF7 antibody (AA 102-269)

Catalog No. ABIN204802

This Mouse Monoclonal antibody specifically detects TCF7 in WB, ELISA, IHC and ICC. It exhibits reactivity toward Human.

Quick Overview for TCF7 antibody (AA 102-269) (ABIN204802)

Target: TCF7 (Transcription Factor 7 (T-Cell Specific, HMG-Box) (TCF7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TCF7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-TCF7 Antibody

Binding Specificity: AA 102-269

Specificity: Recognizes multiple splice isoforms of TcF 1, Mr of 26kD, 32kD, 38kD, 42kD and 48kD. Species cross-reactivity: Human, not mouse.

Purification: Protein G purified

Immunogen: Maltose binding protein (MBP) fusion protein corresponding to residues 102-269 of human TcF 1A splice variant. The epitope maps to a region just N-terminal to the High Mobility Group (HMG) box.

Type of Immunogen: Fusion protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, ICC, IHC, WB

Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 100 μg of mouse IgG1 in 100 μLof 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide prior to addition of glycerol to 30 %.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for TCF7

Target: TCF7 (Transcription Factor 7 (T-Cell Specific, HMG-Box) (TCF7))

Alternative Name: TCF-1 / TCF7

Background: Name/Gene ID: TCF7
Family: Transcription factor

Synonyms: TCF7, TCF-7, TCF1, Transcription factor 7, T-cell factor 1, TCF-1

Gene ID: 6932

UniProt: P36402

Pathways: WNT Signaling