TIMP1 antibody

Catalog No. ABIN204833

The Rabbit Polyclonal anti-TIMP1 antibody has been validated for WB, IHC, ELISA and IHC (pfa). It is suitable to detect TIMP1 in samples from Human, Rat, Rabbit, Horse, Sheep, Cow and Ferret.

Quick Overview for TIMP1 antibody (ABIN204833)

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Reactivity: Human, Rat, Rabbit, Horse, Sheep, Cow, Ferret

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIMP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraformaldehyde) (IHC (pfa))

Product Details anti-TIMP1 Antibody

Specificity: Recognizes human TIMP (Tissue Inhibitor of Metalloproteinase). Reacts with native and denatured TIMP and TIMP in and out of enzyme/inhibitor complex. Does not cross-react with other human proteins tested. Not known to inhibit biological activity.

Purification: Purified

Immunogen: Full length purified human fibroblast TIMP (Tissue Inhibitor of Metalloproteinase).

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (5 μg/mL), IHC, IHC-Pfa, WB (5 - 10 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Western Blot: 5-10 μg/mL. Recognizes a band at ~28-30kD in reduced samples. Immunohistochemistry: 5-20 μg/mL using frozen and 4 % PFA-fixed frozen sections, enzymatic detection. ELISA: 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for TIMP1

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Alternative Name: TIMP1

Background: Name/Gene ID: TIMP1

Synonyms: TIMP1, CLGI, EPA, HCI, Metalloproteinase inhibitor 1, TIMP-1, Collagenase inhibitor

Gene ID: 7076

UniProt: P01033