RANGAP1 antibody (N-Term)

Catalog No. ABIN209212

This Mouse Monoclonal antibody specifically detects RANGAP1 in WB, IF and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RANGAP1 antibody (N-Term) (ABIN209212)

Target: RANGAP1 (Ran GTPase Activating Protein 1 (RANGAP1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This RANGAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Product Details anti-RANGAP1 Antibody

Binding Specificity: N-Term

Specificity: Can be used to specifically detect both the unmodified (~70kD) form and the GMP1 (SUMO-1) conjugated (~90kD) form of the RanGAP-1 protein. The ~70kD form of RanGAP-1 is cytoplasmic, whereas the ~90kD form is associated predominately with the nuclear envelope where it is localized to the nuclear pore complexes (NPCs). Species cross-reactivity: Human and rat. Lysates Tested: Rat liver nuclear envelopes, total lysates derived from NIH 3T3 and HeLa cells.

Purification: Protein A purified

Immunogen: A fusion protein consisting of the first 203 amino-terminal residues of the mouse RanGAP-1 protein.

Type of Immunogen: Fusion protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (1 μg/mL), WB (0.1 - 1 μg/mL)

Usage: Suitable for use in Immunofluorescence, ELISA and Western Blot. Immunofluorescence: 1 μg/mL. ELISA: 0.1-1 μg/mL. Western Blot: 0.1-1 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for RANGAP1

Target: RANGAP1 (Ran GTPase Activating Protein 1 (RANGAP1))

Alternative Name: RANGAP1

Background: Name/Gene ID: RANGAP1

Synonyms: RANGAP1, Fug1, KIAA1835, SD, Segregation distortion, Segregation distorter homolog

Gene ID: 5905

UniProt: P46060

Pathways: M Phase, Protein targeting to Nucleus