Histone H1 antibody (hyperphosphorylated)

Catalog No. ABIN211181

The Rabbit Polyclonal anti-Histone H1 antibody has been validated for WB and ICC. It is suitable to detect Histone H1 in samples from Human.

Quick Overview for Histone H1 antibody (hyperphosphorylated) (ABIN211181)

Target: Histone H1

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Product Details anti-Histone H1 Antibody

Binding Specificity: hyperphosphorylated

Specificity: Recognizes phosphorylated histone H1 (32kD). Additional proteins (Mr 66 and 100kD) were detected. Species cross-reactivity: human, bovine.

Purification: Protein A purified

Immunogen: Hyper-phosphorylated isoform of Tetrahymena Histone H1.

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: ICC (5 - 10 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunocytochemistry. Proteins were visualized using a goat anti-rabbit secondary antibody labeled with HRP. A chemiluminescence detection system was used. Immunocytochemistry: 5-10 μg/mL showed positive immunostaining for Histone H1 in mitotic HeLa cells fixed with 95 % ethanol, 5 % glacial acetic acid.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Histone H1

Target: Histone H1