ACTN1 antibody

Catalog No. ABIN211332

This anti-ACTN1 antibody is a Mouse Monoclonal antibody detecting ACTN1 in WB, IHC, ICC, IP and IHC (fro). Suitable for Human, Mouse and Rabbit.

Quick Overview for ACTN1 antibody (ABIN211332)

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Reactivity: Human, Mouse, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACTN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ACTN1 Antibody

Specificity: Recognizes human alpha-actinin 1 isoform (non-muscle) at 100kD. Species cross-reactivity: Mouse and rabbit. Species sequence homology: Human alpha actinin 2 - 77 % (muscle, myocardium).

Purification: Protein A purified

Immunogen: Human alpha-actinin.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ICC, IHC, IHC-Fr, IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Not suitable for use in Immunoprecipitation. Western Blot: 0.5-2 μg/mL. Detects alpha-Actinin in RIPA lysates from human A431 cells. Immunocytochemistry/Immunohistochemistry: 15 μg/mL. Reported positive immunostaining for alpha-Actinin in MRC-5 cells fixed with methanol and also in frozen sections.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 1 mg/mL BSA, 0.05 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for ACTN1

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Alternative Name: ACTN1

Background: Name/Gene ID: ACTN1

Synonyms: ACTN1, Alpha-actinin-1, Actinin 1 smooth muscle, Actinin, alpha 1, F-actin cross-linking protein, Non-muscle alpha-actinin-1, ASMA

Gene ID: 87

UniProt: P12814

Pathways: Cell-Cell Junction Organization