beta Actin antibody (N-Term)

Catalog No. ABIN215582

The Rabbit Polyclonal anti-beta Actin antibody has been validated for WB, IF, ICC and IP. It is suitable to detect beta Actin in samples from Human, Mouse, Rat and Monkey.

Quick Overview for beta Actin antibody (N-Term) (ABIN215582)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-beta Actin Antibody

Binding Specificity: N-Term

Specificity: Recognizes human beta Actin. Species cross-reactivity: mouse, rat, monkey. Expected to cross-react with a wide range of species due to sequence homology.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from the N-terminus of human beta Actin. Cellular Localization: Cytoplasmic.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IF (1:50), IP, WB (1:500 - 1:2500)

Usage: Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation and Immunofluorescence. Western Blot: 1:500-1:2500. Can be blocked with beta Actin peptide (aa2-15). At higher concentrations, other bands can be detected. Note: May be used as a cell extract loading control. Immunofluorescence: 1:50. Positive control: HeLa whole cell lysate and 3T3 cells (mouse).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-citrate/phosphate buffer,  pH 7-8, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: ACTB / Beta Actin

Background: Name/Gene ID: ACTB

Synonyms: ACTB, Actin, cytoplasmic 1, Beta-actin, Beta actin, BRWS1, Actin, beta, Beta cytoskeletal actin, PS1TP5-binding protein 1, PS1TP5BP1

Gene ID: 60

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction