alpha Actinin 4 antibody (AA 28-38)

Catalog No. ABIN215583

This anti-alpha Actinin 4 antibody is a Rabbit Polyclonal antibody detecting alpha Actinin 4 in WB, IHC, IP, IHC (p) and IHC (fro). Suitable for Human, Mouse, Rat, Monkey, Dog and Cow.

Quick Overview for alpha Actinin 4 antibody (AA 28-38) (ABIN215583)

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Reactivity: Human, Mouse, Rat, Monkey, Dog, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Actinin 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-alpha Actinin 4 Antibody

Binding Specificity: AA 28-38

Specificity: Recognizes human actinin-4. Does not cross-react with actinin-1, -2 or -3. Species cross-reactivity: mouse, rat, porcine and Chinese hamster.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine (100%) Chicken (91%) Pufferfish (82%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to aa 28-38 (MGDYMAQEDDW) of human and rat actinin-4 and to aa 29-39 of mouse actinin-4. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine (100%), Chicken (91%), Pufferfish (82%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-Fr (0.1 - 0.25 μg/mL), IHC-P, IP (25 μg/mL), WB (0.1 - 0.25 μg/mL)

Usage: Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Immunohistochemistry: 1 μg/mL on frozen sections. Also suitable on paraffin sections. Immunoprecipitation: 25 μg/mL. Western Blot: 0.1-0.25 μg/mL. Positive control: A0761-04A Platelet Protein, Human.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 1 mg/mL BSA, 0.01 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for alpha Actinin 4

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Alternative Name: ACTN4

Background: Name/Gene ID: ACTN4

Synonyms: ACTN4, ACTININ-4, Actinin, alpha 4, Actinin alpha4 isoform, FSGS1, F-actin cross-linking protein, Non-muscle alpha-actinin 4, Alpha-actinin-4, FSGS

Gene ID: 81

UniProt: O43707

Pathways: Proton Transport