HPV16 E7 antibody (Middle Region)

Catalog No. ABIN2173795

This Rabbit Polyclonal antibody specifically detects HPV16 E7 in WB, IF (p), ELISA, FACS, IF (cc), IHC (p), IHC (fro) and ICC. It exhibits reactivity toward Human Papillomavirus 16 (HPV-16) and has been mentioned in 2+ publications.

Quick Overview for HPV16 E7 antibody (Middle Region) (ABIN2173795)

Target: HPV16 E7 (HPV-16 E7) (Human Papilloma Virus 16 E7 (HPV-16 E7))

Reactivity: Human Papillomavirus 16 (HPV-16)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HPV16 E7 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-HPV16 E7 Antibody

Binding Specificity: Middle Region

Specificity: This antibody may also detect HPV35-E7

Cross-Reactivity: Human, Virus

Cross-Reactivity (Details): HPV16

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from middle of HPV16-E7

Isotype: IgG

Application Details

Application Notes: WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-HPV16 E7 antibody (ABIN2173795)

Liu, Murray-Stewart, Casero, Kagiampakis, Jin, Zhang, Wang, Che, Tong, Ke, Jiang, Wang, Wan: "Targeting hexokinase 2 inhibition promotes radiosensitization in HPV16 E7-induced cervical cancer and suppresses tumor growth." in: International journal of oncology, Vol. 50, Issue 6, pp. 2011-2023, (2017) (PubMed).

Shao, Sun, Wang, Chung, Du, Wang, Qiu, Wang, Wu: "HPV16 E6/E7 upregulates HIF-2α and VEGF by inhibiting LKB1 in lung cancer cells." in: Tumour biology, Vol. 39, Issue 7, pp. 1010428317717140, (2017) (PubMed).

Target Details for HPV16 E7

Target: HPV16 E7 (HPV-16 E7) (Human Papilloma Virus 16 E7 (HPV-16 E7))

Target Type: Viral Protein

Background:

Synonyms: Protein E7, E7

Background: Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Plays also a role in the inhibition of both antiviral and antiproliferative functions of host inteferon alpha.

Gene ID: 1489079

UniProt: P03129