Podocin antibody (AA 231-330)

Catalog No. ABIN2178520

This Rabbit Polyclonal antibody specifically detects Podocin in WB, FACS, IF (cc), IF (p), ELISA, IHC (fro) and IHC (p). It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 2+ publications.

Quick Overview for Podocin antibody (AA 231-330) (ABIN2178520)

Target: Podocin (NPHS2) (Nephrosis 2, Idiopathic, Steroid-Resistant (Podocin) (NPHS2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Podocin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Podocin Antibody

Binding Specificity: AA 231-330

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Podocin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Podocin antibody (ABIN2178520)

Jayachandran, Lugo, Heiling, Miller, Rule, Lieske: "Extracellular vesicles in urine of women with but not without kidney stones manifest patterns similar to men: a case control study." in: Biology of sex differences, Vol. 6, pp. 2, (2015) (PubMed).

Zhang, Guo, Song, Zhou: "Vitamin D prevents podocyte injury via regulation of macrophage M1/M2 phenotype in diabetic nephropathy rats." in: Endocrinology, Vol. 155, Issue 12, pp. 4939-50, (2014) (PubMed).

Target Details for Podocin

Target: Podocin (NPHS2) (Nephrosis 2, Idiopathic, Steroid-Resistant (Podocin) (NPHS2))

Alternative Name: Podocin

Background:

Synonyms: PDCN, SRN1, Podocin, NPHS2

Background: Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.

Gene ID: 7827

UniProt: Q9NP85