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Surface Layer Protein (AA 301-400) antibody (Biotin)

Reactivity: Bacteria, Lactobacillus acidophilus, Lactobacillus amylovorus, Lactobacillus crispatus, Lactobacillus gallinarum, Lactobacillus helveticus ELISA, IHC (fro), IHC (p) Host: Rabbit Polyclonal Biotin
Catalog No. ABIN2179505
  • Target
    Surface Layer Protein
    Binding Specificity
    AA 301-400
    Reactivity
    Bacteria, Lactobacillus acidophilus, Lactobacillus amylovorus, Lactobacillus crispatus, Lactobacillus gallinarum, Lactobacillus helveticus
    Host
    • 14
    Rabbit
    Clonality
    • 14
    Polyclonal
    Conjugate
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Biotin
    Application
    • 13
    • 13
    • 2
    • 2
    • 2
    ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Cross-Reactivity
    Bacteria
    Cross-Reactivity (Details)
    L.acidophilus, L.crispatus, L.helveticus, L.amylovorus, L.gallinarum
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from L, acidophilus S-layer protein
    Isotype
    IgG
  • Application Notes
    IHC-P 1:200-400
    IHC-F 1:100-500
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C for 12 months.
    Expiry Date
    12 months
  • Target
    Surface Layer Protein
    Background

    Synonyms: S-layer, Surface layer, surface layer protein, Slp, SLAP_LACAC, S-layer protein, slpA, sl pH , Lactobacillus surface layer protein,

    Background: One of the most fascinating cell envelope structures in prokaryotic organisms is two-dimensional arrays of protein or glycoprotein subunits, termed S-layers (Surface Layers). They are composed of numerous identical subunits forming a symmetric, porous, lattice-like layer that completely covers the cell surface. The subunits are held together and attached to cell wall carbohydrates by non-covalent interactions, and they spontaneously reassemble in vitro by an entropy-driven process. In lactobacilli, S-layer proteins have been detected on many but not all species. Lactobacillus S-layer proteins differ from those of other bacteria in their smaller size and high predicted pI. The positive charge in Lactobacillus S-layer proteins is concentrated in the more conserved cell wall binding domain, which can be either N- or C-terminal depending on the species. The more variable domain is responsible for the self-assembly of the monomers to a periodic structure. The biological functions of Lactobacillus S-layer proteins are poorly understood, but in some species S-layer proteins mediate bacterial adherence to host cells or extracellular matrix proteins or have protective or enzymatic functions. Lactobacillus S-layer proteins show potential for use as antigen carriers in live oral vaccine design because of their adhesive and immunomodulatory properties and the general non-pathogenicity of the species.

    Gene ID
    3252774
    UniProt
    P35829
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