M-Csf Responsive Cells antibody

Catalog No. ABIN2191838

Product Details

Target: M-Csf Responsive Cells

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Sterility: 0.2 μm filtered

Clone: ER-MP58

Application Details

Application Notes: For immunohistology and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References

Cook, Vlahos, Massa, Braine, Lenzo, Turner, Way, Hamilton: "The effect of tissue type-plasminogen activator deletion and associated fibrin(ogen) deposition on macrophage localization in peritoneal inflammation." in: Thrombosis and haemostasis, Vol. 95, Issue 4, pp. 659-67, (2006) (PubMed).

Sunderkötter, Nikolic, Dillon, Van Rooijen, Stehling, Drevets, Leenen: "Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 7, pp. 4410-7, (2004) (PubMed).

Chan, Leenen, Bertoncello, Nishikawa, Hamilton: "Macrophage lineage cells in inflammation: characterization by colony-stimulating factor-1 (CSF-1) receptor (c-Fms), ER-MP58, and ER-MP20 (Ly-6C) expression." in: Blood, Vol. 92, Issue 4, pp. 1423-31, (1998) (PubMed).

de Bruijn, Ploemacher, Mayen, Voerman, Slieker, van Ewijk, Leenen: "High-level expression of the ER-MP58 antigen on mouse bone marrow hematopoietic progenitor cells marks commitment to the myeloid lineage." in: European journal of immunology, Vol. 26, Issue 12, pp. 2850-8, (1997) (PubMed).

Target Details

Target: M-Csf Responsive Cells

Background: The monoclonal antibody ER-MP58 recognizes an antigen present on all mouse macrophage colony- stimulating factor (M-CSF) responsive cells in the bone marrow, including the earliest colony-forming myeloid progenitors, as well as by the majority of other myeloid precursors. The antigen is detected on a broad development range of macrophage precursor cells to the monocytic level, but also on granulocytes. Expression is rapidly lost upon maturation beyond the monocytic stage. The antigen disappears in the course of macrophage differentiation. Furthermore the antigen is clearly different from commonly used myeloid markers as Mac-1, F4/80, and Gr-1. The monoclonal antibody ER-MP58 is very useful for the identification of mouse myeloid hematopoietic islands in various organs, and for embryonic studies. Cells committed to the myeloid lineage can be separated from progenitor cells with other differentiation capacities by means of multiparameter cell sorting using monoclonal antibody ER-MP58 in combination with monoclonal antibody ER-MP12 (HM1084) and ER-MP-20 (HM1082).