Activated c1s antibody

Catalog No. ABIN2191987

This Mouse Monoclonal antibody specifically detects in IA, IHC (fro), FACS, InhA, IP, IHC (p) and WB. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for Activated c1s antibody (ABIN2191987)

Target: Activated c1s

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunoassay (IA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Inhibition Assay (InhA), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Clone: M241

Product Details

Sterility: 0.2 μm filtered

Application Details

Application Notes: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of biological activity dilutions have to be made according to the amounts C1s to be inactivated.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References

Matsumoto, Nagaki, Kitamura, Kuramitsu, Nagasawa, Seya: "Probing the C4-binding site on C1s with monoclonal antibodies. Evidence for a C4/C4b-binding site on the gamma-domain." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2743-50, (1989) (PubMed).

Target Details

Target: Activated c1s

Background: Monoclonal antibody M241 reacts with an epitope on human C protein activated C1s, a subcomponent of the first component of C (C1). Activated C1s is a glycosylated single-polypeptide zymogen of 85 kD. Activation of the proenzyme C1s occurs through cleavage by the active form of C1r. The activated protease, activated C1s, consists of a disulfide-linked H chain and a L chain. Activated C1s is a serine protease and its catalytic site is located in the L chain. Activation of the classical C pathway is triggered by activated C1s which cleaves C4 and C2 to form the C3 convertase, C4bC2a. The epitope recognised by the antibody M241 is domain IV and/or V of the gamma-domain of activated C1s. Monoclonal antibody M241 blocks C4 activation and C4 binding to activated C1s. The antibody binds specifically to the active centre of C1s.