Caveolin-1 antibody

Catalog No. ABIN2192105

The Mouse Monoclonal anti-Caveolin-1 antibody has been validated for WB. It is suitable to detect Caveolin-1 in samples from Rat. There are 2+ publications available.

Quick Overview for Caveolin-1 antibody (ABIN2192105)

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caveolin-1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 7C8

Product Details anti-Caveolin-1 Antibody

Sterility: 0.2 μm filtered

Application Details

Application Notes: For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive Adipocytes (3T3-L1 adipocytes) control Negative Cytoplasmic extracts of adipocytes control

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-Caveolin-1 antibody (ABIN2192105)

Salani, Passalacqua, Maffioli, Briatore, Hamoudane, Contini, Cordera, Maggi: "IGF-IR internalizes with Caveolin-1 and PTRF/Cavin in HaCat cells." in: PLoS ONE, Vol. 5, Issue 11, pp. e14157, (2010) (PubMed).

Thoidis, Kotliar, Pilch: "Immunological analysis of GLUT4-enriched vesicles. Identification of novel proteins regulated by insulin and diabetes." in: The Journal of biological chemistry, Vol. 268, Issue 16, pp. 11691-6, (1993) (PubMed).

Target Details for Caveolin-1

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Alternative Name: Caveolin-1

Background: The monoclonal antibody 7C8 recognizes rat caveolin-1, a membrane protein of ~22 kDa. Caveolae are sphingomyelin/cholesterol-rich membrane domains first discovered as membrane invaginations on the surface of endothelial and epithelial cells. Caveolae are present in most cells, but are especially abundant in adipocytes. In addition to caveolins only two major protein components of caveolae were identified, namely the semicarbazide sensitive amine oxidase (SSAO) and the scavenger receptor CD36. Caveolin cycles between the plasma membrane and intracellular compartments via the endocytotic pathway. Caveolin is involved in the rapid intracellular transport of newly synthesized cholesterol from the ER directly to the caveolae. Caveolin plays an important role in multiple signaling pathways, molecular transport and cellular proliferation and differentiation. Caveolin binds to endothelial nitric oxide synthase leading to enzyme inhibition. Furthermore caveolin is a candidate tumor suppressor gene in many tumors. The specific functions of caveolin-1/caveolae are highly cell and context dependent. The monoclonal antibody 7C8 recognizes caveolin-1α as well as caveolin-1β, which are present in many tissues, like aorta, heart, muscle, lung, adipose white, brown and epidydimal fat. The monoclonal antibody 7C8 can be used to immuno-isolate caveolae. Immunogen GLUT4-containing vesicles immunoadsorbed from low density microsomes of rat adipocytes (Sprague Dawley) (Ref 4)

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals