CD55 antibody

Catalog No. ABIN2192124

This anti-CD55 antibody is a Mouse Monoclonal antibody detecting CD55 in WB and IHC (fro). Suitable for Rat. This Primary Antibody has been cited in 2+ publications.

Quick Overview for CD55 antibody (ABIN2192124)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD55 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RDII-24

Product Details anti-CD55 Antibody

Sterility: 0.2 μm filtered

Application Details

Application Notes: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-CD55 antibody (ABIN2192124)

Harris, Williams, Linton, Morgan: "Coupling complement regulators to immunoglobulin domains generates effective anti-complement reagents with extended half-life in vivo." in: Clinical and experimental immunology, Vol. 129, Issue 2, pp. 198-207, (2002) (PubMed).

Spiller, Harris, Morgan: "Efficient generation of monoclonal antibodies against surface-expressed proteins by hyperexpression in rodent cells." in: Journal of immunological methods, Vol. 224, Issue 1-2, pp. 51-60, (1999) (PubMed).

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: Complement Decay Accelerating Factor

Background: The monoclonal antibody RDII-24 recognizes complement decay accelerating factor (DAF), also designated as CD55.. Cells express on their surface several proteins which protect against complement attack, namely C receptor I (CR1), decay accelerating factor (DAF), membrane cofactor protein (MCP) and CD59. CR1, DAF and MCP regulate the activation pathways of complement by either accelerating decay of the C3 and C5 convertase (CR1, DAF), or acting as cofactors for the serine protease factor I, which cleaves and irreversibly inactivates C3b (CR1, MCP). Rat DAF (CD55) is a 60 kDa transmembrane protein that binds C3b and C4b to inhibit formation and half- life of the C3 convertases. It belongs to the receptors of complement activation (RCA) family. DAF is broadly distributed among cells in contact with plasma complment proteins, including both haematopoietic and nonhaematopoietic cells. Although DAF does not have an essential role in controlling hemolysis of erythrocytes, it has an important role in regulation of the deposition of C3 on nucleated cells. Together with other complement regulators DAF protects self cells from autologous complement- mediated injury. DAF cooperates with CD46 in circumventing autologous C3 deposition, while CD59 inhibits the pathway at the critical end-point. Aliases DAF, CD55 Immunogen NIH-3T3 cells expressing GPI-anchored rat DAF

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity