Occludin antibody

Catalog No. ABIN2192203

This Guinea Pig Polyclonal antibody specifically detects Occludin in WB, IF and IHC (fro). It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for Occludin antibody (ABIN2192203)

Target: Occludin (OCLN)

Reactivity: Human

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This Occludin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Occludin Antibody

Sterility: 0.2 μm filtered

Application Details

Application Notes: For immunohistology, immunfluorescence and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50..

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-Occludin antibody (ABIN2192203)

Van Itallie, Anderson: "Occludin confers adhesiveness when expressed in fibroblasts." in: Journal of cell science, Vol. 110 ( Pt 9), pp. 1113-21, (1997) (PubMed).

Target Details for Occludin

Target: Occludin (OCLN)

Alternative Name: Occludin

Background: The polyclonal antibody reacts with human occludin, an integral membrane protein specifically associated with tight junctions. These specialized regions of the plasma membrane prevent the diffusion of dissolved molecules between adjacent epithelial cells, seal off body cavities such as intestine or stomach lumen and prevent the diffusion of membrane proteins and glycolipids between the apical and basolateral regions of the plasma membrane. Occludin is localized at tight junctions of both epithelial and endothelial cells and is highly expressed in kidney and has not been detected in testis. On the cytoplasmic surface of occludin, a domain within the C-terminal 150 residues has been shown to bind directly to the well-characterized tight junction protein zona occludens 1 (ZO-1). The antibody is generated against recombinant human occludin (aa 358-504). The polyclonal anti- human occludin antibody cross reacts with rat, mouse and dog occludin.

Pathways: Cell-Cell Junction Organization, Hepatitis C