ACTC1 antibody (N-Term)

Catalog No. ABIN224333

The Mouse Monoclonal anti-ACTC1 antibody has been validated for WB, IHC, ELISA, IHC (p) and IHC (fro). It is suitable to detect ACTC1 in samples from Human, Rabbit, Chicken and Cow. There is 1 publication available.

Quick Overview for ACTC1 antibody (N-Term) (ABIN224333)

Target: ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))

Reactivity: Human, Rabbit, Chicken, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACTC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ACTC1 Antibody

Binding Specificity: N-Term

Specificity: Recognizes fetal cardiac actin. Represents an excellent marker for cardiac tissue, it discriminates fetal (cardiac) a-actin from all other actin isoforms. Shows no cross-reaction with other actin isoforms present in skeletal and smooth muscle, provided that stringent experimental conditions have been applied. Species cross-reactivity: Human, bovine, rabbit, chicken.

Purification: Protein G purified

Immunogen: Synthetic N-terminus decapeptide of cardiac isoform of actin.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (1:500), IHC, IHC-Fr, IHC-P, WB (1:1000)

Usage: Suitable for use in Western Blot, ELISA and Immunohistochemistry. Immunohistochemistry: 1:10. Frozen and paraffin sections. Note: It is necessary to include 0.5 M sodium chloride in all washing buffers for application on frozen tissue. This is not necessary for paraffin sections. Western Blot: 1:1000 (using the ECL-enhanced procedure). ELISA: 1:500. Note: It is necessary to include 1 M sodium chloride for application in Western blot and ELISA to enhance specificity.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile ddH2O

Concentration: Lot specific

Buffer: Lyophilized powder from PBS,  pH 7.4, 0.5 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing

Storage: -20 °C

Storage Comment: Reconstituted or lyophilized: store at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid freeze-thaw cycles.

References for anti-ACTC1 antibody (ABIN224333)

Itier, Ret, Viale, Sweet, Bangari, Caron, Le-Gall, Bénichou, Leonard, Deleuze, Orsini: "Effective clearance of GL-3 in a human iPSC-derived cardiomyocyte model of Fabry disease." in: Journal of inherited metabolic disease, (2014) (PubMed).

Target Details for ACTC1

Target: ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))

Alternative Name: ACTC1 / Alpha Cardiac Actin

Background: Name/Gene ID: ACTC1

Synonyms: ACTC1, Actin, alpha cardiac muscle 1, Actin, alpha, cardiac muscle, Actin, alpha, cardiac muscle 1, Alpha-cardiac actin, CMH11, CMD1R, ACTC, LVNC4, ASD5

Gene ID: 70

UniProt: P68032

Pathways: Myometrial Relaxation and Contraction