APC antibody (AA 1-433)

Catalog No. ABIN224804

The Mouse Monoclonal anti-APC antibody has been validated for WB, IHC and IHC (fro). It is suitable to detect APC in samples from Human.

Quick Overview for APC antibody (AA 1-433) (ABIN224804)

Target: APC (Adenomatous Polyposis Coli (APC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-APC Antibody

Binding Specificity: AA 1-433

Specificity: Recognizes the N-terminal region of the human APC protein. Epitope Mapping: Performed by differential in vitro expression of the N-terminal region of the APC gene by using the protein truncation test. Found to bind to APC in the region between aa135-422 (exon 2-3). Species cross-reactivity: Does not cross-react with mouse.

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to aa1-433 N-terminal fragment of human APC (Adenomatous Polyposis Coli gene Chr 5q) (coupled to maltose binding protein). Cellular localization: cytoplasmic and nuclear.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-Fr, WB

Usage: Suitable for use in Immunohistochemistry and Western Blot. Immunohistochemistry: Acetone fixed, frozen sections. Positive control: Colon cell line HCT116, SW480 and SW837 extracts.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 40 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for APC

Target: APC (Adenomatous Polyposis Coli (APC))

Alternative Name: APC

Background: Name/Gene ID: APC

Synonyms: APC, Adenomatosis polyposis coli, Adenomatous polyposis coli, BTPS2, Deleted in polyposis 2.5, DP2, DP2.5, FPC, GS, Protein APC, PPP1R46, DP3

Gene ID: 324

UniProt: P25054

Pathways: WNT Signaling, Stem Cell Maintenance, Cell-Cell Junction Organization