Orexin antibody (N-Term)

Catalog No. ABIN224841

This anti-Orexin antibody is a Rabbit Polyclonal antibody detecting Orexin in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for Orexin antibody (N-Term) (ABIN224841)

Target: Orexin (OX)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Orexin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Orexin Antibody

Binding Specificity: N-Term

Specificity: Reacts with full length Orexin-B. The epitope is not found in Orexin-A. Since Orexins-A and Orexin-B are derived from an unprocessed pre-pro orexins (130 aa), the anti-Orexin-B antibodies may also react with pre-pro orexins. Species cross-reactivity: Mouse, rat and human. The immunogen peptide has 92 % homology with rat and human Orexin-B.

Purification: Affinity purified

Immunogen: A 13 amino acid peptide mapping near the amino terminus of mouse Orexin-B.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:100000), WB (1 - 10 μg/mL)

Usage: Western blot: 1-10 μg/mL using Chemiluminescence technique ELISA: 1:10000-1:100000. Immunohistochemistry: Not tested. It is recommended that the antibody be tried at 2-20 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % BSA, 0.02 % sodium merthiolate.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Orexin

Target: Orexin (OX)

Alternative Name: HCRT / Orexin

Background: Name/Gene ID: HCRT

Synonyms: HCRT, PPORX, OX, Hypocretin, NRCLP1, Orexin

Gene ID: 3060

UniProt: O43612

Pathways: Synaptic Membrane, Feeding Behaviour