RAD9A antibody (AA 1125-1139)

Catalog No. ABIN225443

The Rabbit Polyclonal anti-RAD9A antibody has been validated for WB and ELISA. It is suitable to detect RAD9A in samples from Saccharomyces cerevisiae.

Quick Overview for RAD9A antibody (AA 1125-1139) (ABIN225443)

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD9A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RAD9A Antibody

Binding Specificity: AA 1125-1139

Specificity: Detects Saccharomyces cerevisiae Rad9, a protein involved in the DNA damage checkpoint. In S. cerevisiae this checkpoint is mainly controlled by the PI(3) kinase-like kinase (PIKK) Mec1, which is activated in response to DNA damage. Activated Mec1 hyperphosphorylates Rad9, which in turn triggers the binding of Rad53, the ortholog of mammalian Chk2. Activated Rad53 specifically targets substrates required for cell cycle arrest, gene expression and efficient DNA repair. Mutations affecting Rad9 impair checkpoint induced cell-cycle arrest and increase genomic instability. Is pan reactive with both the phosphorylated and non-phosphorylated forms of this protein.

Purification: Purified

Immunogen: Synthetic peptide corresponding to aa 1125-1139 of yeast Rad9 protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:2000 - 1:4000), WB (1:50 - 1:250)

Usage: Western Blot: LS-C42615 detects a band of approximately 60kD.

Comment:

Target Species of Antibody: Yeast

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for RAD9A

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Alternative Name: RAD9A / RAD9

Background: Name/Gene ID: RAD9A

Synonyms: RAD9A, HRAD9, RAD9, RAD9 homolog, RAD9 (S. pombe) homolog, RAD9 homolog A (S. pombe)

Gene ID: 5883

UniProt: Q99638

Pathways: Positive Regulation of Response to DNA Damage Stimulus