N-Cadherin antibody (AA 802-819)

Catalog No. ABIN225503

The Mouse Monoclonal anti-N-Cadherin antibody has been validated for WB. It is suitable to detect N-Cadherin in samples from Human, Mouse, Rat, Chicken, Cow, Rabbit, Dog, Horse, Sheep and Bat.

Quick Overview for N-Cadherin antibody (AA 802-819) (ABIN225503)

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reactivity: Human, Mouse, Rat, Chicken, Cow, Rabbit, Dog, Horse, Sheep, Bat

Host: Mouse

Clonality: Monoclonal

Conjugate: This N-Cadherin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-N-Cadherin Antibody

Binding Specificity: AA 802-819

Specificity: Recognizes human N-Cadherin. Species cross-reactivity: Rat, mouse, and chicken.

Predicted Reactivity: Percent identity by BLAST analysis: Galago, Mouse, Rat, Shrew, Sheep, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Opossum, Turkey, Chicken, Armadillo, Platypus, Lizard (100%) Human, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Xenopus (94%) Pufferfish (89%) Zebrafish (83%).

Purification: Purified

Immunogen: C-terminal region peptide corresponding to aa802-819 of mouse N-cadherin KPVGIRRLDERPIHAEPQ. Percent identity by BLAST analysis: Galago, Mouse, Rat, Shrew, Sheep, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Opossum, Turkey, Chicken, Armadillo, Platypus, Lizard (100%), Human, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Xenopus (94%), Pufferfish (89%), Zebrafish (83%).

Isotype: IgG1

Application Details

Application Notes: Approved: WB (0.1 - 0.5 μg/mL)

Usage: Suitable for use in Western Blot. Western Blot: 0.1-0.5 μg/mL using total rat brain cells (2-3hours at RT or overnight at 4?C using ECL chemiluminescence detection). Major band at 130kD.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 50 % glycerol, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Alternative Name: CDH2 / N Cadherin

Background: Name/Gene ID: CDH2
Family: Cadherin

Synonyms: CDH2, CDw325, CDHN, Cadherin-2, CD325, CD325 antigen, N Cadherin, N-cadherin, NCAD, Neural cadherin, N-cadherin 1, Neural-cadherin

Gene ID: 1000

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane