APOE antibody

Catalog No. ABIN227091

The Mouse Monoclonal anti-APOE antibody (ABIN227091) specifically detects APOE in WB, IHC, ELISA, IP, IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for APOE antibody (ABIN227091)

Target: APOE (Apolipoprotein E (APOE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APOE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-APOE Antibody

Specificity: Recognizes human Apo E. Recognizes E2, E3 and E4 isoforms of apolipoprotein E. Does not react with mouse and rabbit.

Purification: Protein A purified

Immunogen: Synthetic peptide spanning the polymorphic aa position 158 of Apo E.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr, IHC-P (1:50 - 1:500), IP, WB (1:1000 - 1:10000)

Usage: Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Western Blot: 1:1000-1:10000. Immunohistochemistry (Frozen and formalin-fixed paraffin-embedded tissues): 1:1000-1:10000. The staining intensity of formalin-fixed paraffin embedded tissues may be significantly improved by the pretreatment of: 1. Formic acid (70 % ) for 10-30 minutes at RT. 2. HIER (Heat Induced Epitope Retrieval) with buffers.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for APOE

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: APOE / Apolipoprotein E

Background: Name/Gene ID: APOE

Synonyms: APOE, AD2, Apo-E, Apolipoprotein E3, Apolipoprotein E, LDLCQ5, LPG

Gene ID: 348

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism