DRD4 antibody (AA 176-185)

Catalog No. ABIN227849

The Rabbit Polyclonal anti-DRD4 antibody has been validated for WB, ELISA and IHC. It is suitable to detect DRD4 in samples from Rat.

Quick Overview for DRD4 antibody (AA 176-185) (ABIN227849)

Target: DRD4 (Dopamine Receptor D4 (DRD4))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DRD4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-DRD4 Antibody

Binding Specificity: AA 176-185

Specificity: Recognizes rat D4 Dopamine Receptor (Ac-176-185) 100 % D4 Dopamine Receptor 60 % D1 Dopamine Receptor (9-21) 0 % D1 Dopamine Receptor 0 % D2 Dopamine Receptor (272-282) 0 % D2 Dopamine Receptor 0 % D3 Dopamine Receptor (2-10) 0 % D3 Dopamine Receptor 0 % D5 Dopamine Receptor (23-35) 0 % D5 Dopamine Receptor 0 %.

Purification: Delipidated and defibrinated

Immunogen: D4 (Ac-176-185) covalently attached to a carrier protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, WB

Usage: Suitable for immunocytochemical detection of the D4 dopamine receptor. Characterized by immunocytochemical, western immunoblot and ELISA techniques. This antiserum has been found to stain specific cells in various regions of PLP fixed rat brain sections at a dilution of 1:10000. This includes the glomeruli of the olfactory bulb, staining of cortex layer 4 and dendritic staining in cortex layers 2 and 3. In layer 5 of the cortex, apical dendrites of many neurons were stained. In the hippocampus, diffuse immunoreactivity was observed in the molecular layer of the dentate gyrus, with fine dots associated with many neurons in the granule cell layer. Western immunoblots using whole rat brain homogenate resulted in a single wide band being detected at ~48-53kD at 1:500 dilution.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for DRD4

Target: DRD4 (Dopamine Receptor D4 (DRD4))

Alternative Name: DRD4 / Dopamine Receptor D4

Background: Name/Gene ID: DRD4
Subfamily: Dopamine
Family: GPCR

Synonyms: DRD4, D(4) dopamine receptor, D4DR, D(2C) dopamine receptor, D4 dopamine receptor, Dopamine receptor D4, Dopamine 4 receptor, Dopamine D4 receptor

Gene ID: 1815

UniProt: P21917

Pathways: cAMP Metabolic Process, Synaptic Membrane, Proton Transport, Photoperiodism, Negative Regulation of Transporter Activity