GNAS antibody (AA 1028-1037)

Catalog No. ABIN228262

This Rabbit Polyclonal antibody specifically detects GNAS in WB, ICC and IP. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Pig, Horse, Monkey, Bat, Zebrafish (Danio rerio), Hamster, Chicken, Drosophila melanogaster, Insect and Xenopus laevis.

Quick Overview for GNAS antibody (AA 1028-1037) (ABIN228262)

Target: GNAS (GNAS Complex Locus (GNAS))

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig, Horse, Monkey, Bat, Zebrafish (Danio rerio), Hamster, Chicken, Drosophila melanogaster, Insect, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNAS antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-GNAS Antibody

Binding Specificity: AA 1028-1037

Specificity: Recognizes both the large (MW 48-52kD) and small (MW43-45kD) forms of bovine Gs alpha. Does not cross-react with other G proteins.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Seabass, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito (100%).

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to the C-terminal region of bovine Gs alpha (RMHLRQYELL) (KLH). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Seabass, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito (100%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:100), IP, WB (1:500 - 1:1000)

Usage: Western Blot: 1:1000-1:2000 detected both forms of Gs alpha as a doublet in 20 μg of rat brain microsomal preparation. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with LS-C24097 (1:1000). Proteins were visualized using a IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.Immunoprecipitation: 2 μL immunoprecipitates Gs alpha(45kD) from 500 μg of solubilized CHO and mouse brain membrane preparations.Immunocytochemistry: 1:200 detected Gs alpha in 3T3 cells fixed for 3 minutes with 100 % ice-cold acetone.Optimal dilutions to be determined by researcher.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for GNAS

Target: GNAS (GNAS Complex Locus (GNAS))

Alternative Name: GNAS

Background: Name/Gene ID: GNAS

Synonyms: GNAS, AHO, C20orf45, Extra large alphas protein, GSP, GNAS complex locus, GNASXL, NESP, PHP1B, PHP1C, PHP1A, POH, SCG6, XLalphas, GNAS1, GPSA, GSA, NESP55, Protein ALEX, Secretogranin VI

Gene ID: 2778

Pathways: Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Embryonic Body Morphogenesis