CENPA antibody (AA 4-17)

Catalog No. ABIN232755

This anti-CENPA antibody is a Rabbit Polyclonal antibody detecting CENPA in WB. Suitable for Human and Monkey.

Quick Overview for CENPA antibody (AA 4-17) (ABIN232755)

Target: CENPA (Centromere Protein A (CENPA))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CENPA Antibody

Binding Specificity: AA 4-17

Specificity: Recognizes human CENP-A, Mr17kD. Broad species cross-reactivity predicted due to sequence conservation.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%) Gibbon, Rabbit (93%) Nectri (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide (RRRSRKPEAPRRRS) corresponding to amino acids 4-17 of human CENP-A. KLH conjugated. Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%), Gibbon, Rabbit (93%), Nectri (86%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: Suitable for use in Western Blot analysis. Western Blot Analysis: 1:200-1:1000 detected CENP-A in RIPA lysates from HeLa acid extracted histones. 20 μg control histones, were resolved by electrophoresis, transferred to nitrocellulose and probed with 2 μg/mL anti-CENP-A. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 5 mg/mL BSA, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CENPA

Target: CENPA (Centromere Protein A (CENPA))

Alternative Name: CENPA / CENP-A

Background: Name/Gene ID: CENPA

Synonyms: CENPA, Centromere autoantigen A, Centromere-specific histone, CenH3, Centromere protein A, Centromere protein A, 17  kDa, CENP-A, Centromere protein A (17kD)

Gene ID: 1058

UniProt: P49450

Pathways: Chromatin Binding, Maintenance of Protein Location