ASAP3 antibody

Catalog No. ABIN233800

Supplier Product No.: 200-401-990

Product Details anti-ASAP3 Antibody

Target: ASAP3 (ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 3 (ASAP3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASAP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 200-401-990

Supplier: Rockland

Purpose: UPLC1/ASAP3 Antibody

Cross-Reactivity (Details): This protein A purified antibody is directed against human UPLC1/ASAP3 protein.

Characteristics: Synonyms: rabbit anti-UPLC1 Antibody, rabbit anti-ASAP3 Antibody, UPLC1/ASAP3 Antibody, Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3, FLJ20199 antibody, Gm140 antibody, Development and differentiation-enhancing factor-like 1, Protein up-regulated in liver cancer 1, DDEFL1

Purification: The product was purified from monospecific antiserum by protein A chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant human UPLC1/ASAP3 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This protein A purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 100 kDa in size corresponding to UPLC1/ASAP3 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200 - 1:2,000

ELISA Dilution: 1:1,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store UPLC1 / ASAP3 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-ASAP3 antibody (ABIN233800)

Luo, Kong, Wang, Chen, Liu, Wang, Xu, Wang, Yang: "Loss of ASAP3 destabilizes cytoskeletal protein ACTG1 to suppress cancer cell migration." in: Molecular medicine reports, Vol. 9, Issue 2, pp. 387-94, (2014) (PubMed).

Target Details for ASAP3

Target: ASAP3 (ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 3 (ASAP3))

Alternative Name: ASAP3 (ASAP3 Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Anti-UPLC1 (up-regulated in liver cancer 1) / ASAP3 Antibody, also named DDEFL1 (development and differentiation-enhancing factor-like 1) or ASAP3, is a member of the AZAP family of proteins. These proteins catalyze the hydrolysis of GTP bound to ADP-ribosylation factor (Arf) proteins, thereby causing Arf inactivation. For this reason, the ASAPs are generally called ArfGAPs.  The activity of ArfGAPs is dependent on the presence of phosphoinositides and is implicated in cellular processes such as membrane trafficking and remodeling of the actin cytoskeleton.  ASAP3 has been found to be up-regulated in 80 % of the hepatocellular carcinomas examined.  Initial biochemical characterization reveals that ASAP3 shows class-specific GAP activity on Arf proteins, preferring Arf5 over Arf1 and Arf6. ASAP3 antibody has beed developed through the NCI antibody collaboration program and is ideal for Cancer and Signal Transduction research.

Gene ID: 55616, 19071867

UniProt: Q8TDY4