CD13 Myeloid Cell antibody (Biotin)

Catalog No. ABIN234513

This anti- antibody is a Mouse Monoclonal antibody detecting in FACS and IHC (f). Suitable for Human.

Quick Overview for CD13 Myeloid Cell antibody (Biotin) (ABIN234513)

Target: CD13 Myeloid Cell

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 22A5

Product Details

Specificity: Recognizes the (Mr 150-170 kDa) cell surface glycoprotein expressed in a pan-myeloid fashion. This antibody also reacts with osteoclasts in giant cell tumors of bone (osteoclastoma), clear cell chondrosarcoma and aneurysmal bone cysts (1). The CD13 antigen is present on most cells of myeloid origin, including granulocytes and monocytes in normal peripheral blood. CD13 is not expressed on B-cells, T-cells, platelets or erythrocytes. Expression of this antigen is greater on monocytes than on granulocytes.

Characteristics: MAb to CD13 Myeloid Cells, Monoclonal antibody to Human CD13 Myeloid cells Biotin conjugated

Purification: Protein A Chromatography. Tissue culture

Immunogen: A cell suspension containing osteoclasts from osteoclastomas

Isotype: IgG

Application Details

Application Notes: Suitable for use in flow cytometry and immunohistochemistry (acetone-fixed frozen sections). We recommend using 1 µg to stain 1.0 x 106 cells in flow cytometric applications. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M PBS, pH 7.2 containing 2 mM EDTA, 1 % BSA, 0.1 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details

Target: CD13 Myeloid Cell

Alternative Name: CD13 (Myeloid cells)