CD15 (Granulocytes) antibody (Biotin)

Catalog No. ABIN234527

The Mouse Monoclonal anti- antibody has been validated for FACS, IHC (f) and IFA. It is suitable to detect in samples from Human.

Quick Overview for CD15 (Granulocytes) antibody (Biotin) (ABIN234527)

Target: CD15 (Granulocytes)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Indirect Immunofluorescence Assay (IFA)

Clone: TG1

Product Details

Specificity: Recognizes a (Mr 156 kDa) cell surface glycoprotein expressed on virtually all peripheral blood granulocytes.(1,2,3) The CD15 antigen is expressed on approximately 95% of human peripheral blood eosinophils and neutrophils and is present on approximately 20% of mature circulating monocytes. Anti-CD15 has virtually no reactivity with T or B cells.(1,2,3)

Characteristics: MAb to CD15 Granulocytes, Monoclonal antibody to CD15 (Granulocytes) Biotin conjugated

Purification: Ion Exchange Chromatography and gel filtration. Tissue culture

Immunogen: Thymocyte glycoprotein fraction adsorbed to Con A and eluted with 0.1M glucose

Isotype: IgM

Application Details

Application Notes: Suitable for use in flow cytometry, IFA and IHC (acetone-fixed frozen sections). May be used for enumerating mature granulocytes in peripheral blood. We recommend using 1 µg to stain 1.0 x 106 cells in flow cytometric applications. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M PBS, pH7.2 containing 1 % BSA, 0.1 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details

Target: CD15 (Granulocytes)