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Myeloperoxidase antibody

This anti-Myeloperoxidase antibody is a Mouse Monoclonal antibody detecting Myeloperoxidase in WB, ELISA, FACS, IHC (p) and IHC (f). Suitable for Human.
Catalog No. ABIN235905

Quick Overview for Myeloperoxidase antibody (ABIN235905)

Target

See all Myeloperoxidase (MPO) Antibodies
Myeloperoxidase (MPO)

Reactivity

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Human

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This Myeloperoxidase antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone

2C7
  • Specificity

    Recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils. The protein is a multimer of 2 heavy chains (55kD) and two light chains (15kD), the heavy chains being linked by a disulfide bond. Recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. Also recognizes recombinant MPO in Western blots and weakly in ELISA.

    Characteristics

    MAb to Myeloperoxidase, Monoclonal antibody to Human Myeloperoxidase (MPO)

    Purification

    Protein G Chromatography.

    Immunogen

    Human Myeloperoxidase

    Isotype

    IgG1
  • Application Notes

    Suitable for use in Flow cytometry following cell permeabilization (1:50-1:100), immunohistochemistry with frozen sections (1:1.000 - 1:5.000) and paraffin sections (1:500 - 1:1.000), ELISA and Western blot (non-reducing conditions). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    PBS, pH 7.4, 0.09 % Sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid multiple freeze/thaw cycles.
    Centrifuge product if not completely clear after standing at room temperature.
    Prepare working dilution only prior to immediate use.

    Storage

    4 °C/-20 °C

    Storage Comment

    Short-term (up to 2 weeks) store at 2-8 °C. Long term store at -28 °C.

    Expiry Date

    2 weeks
  • Target

    Myeloperoxidase (MPO)

    Pathways

    Chromatin Binding
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