E. Coli, Serotype O/K antibody (FITC)

Catalog No. ABIN237175

Product Details

Target: E. Coli, Serotype O/K

Reactivity: E. coli

Host: Goat

Clonality: Polyclonal

Conjugate: FITC

Application: ELISA, Indirect Immunofluorescence Assay (IFA)

Cross-Reactivity (Details): Many O and K antigenic serotypes of Escherichia coli. Antiserum is not absorbed and may react with related Enterobacteriaceae

Characteristics: Goat anti E. coli (all antigens), Goat antibody to Escherichia coli (E. coli), O and K antigenic serotypes Fluorescein conjugated

Purification: IgG fraction coupled with high purity isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product. Ascites

Immunogen: Mixture of E. coli serotypes

Application Details

Application Notes: Suitable for use in ELISA and direct IFA. Acetone fixation of the antigen source is recommended prior to staining. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M PBS, pH 7.2 containing 10 mg/mL BSA, 0.1 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid multiple freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Short-term (up to 6 months) store at 2-8 °C under subdued light. Long term, aliquot and store at -28 °C.

Expiry Date: 6 months

Target Details

Target: E. Coli, Serotype O/K

Alternative Name: Escherichia Coli (O and K Antigenic Serotypes)