Shigella antibody

Catalog No. ABIN238106

The Rabbit Polyclonal anti-Shigella antibody has been validated for IF. It is suitable to detect Shigella in samples from Shigella.

Quick Overview for Shigella antibody (ABIN238106)

Target: Shigella

Reactivity: Shigella

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Shigella antibody is un-conjugated

Application: Immunofluorescence (IF)

Product Details anti-Shigella Antibody

Cross-Reactivity (Details): Polyvalent for Shigella antigens. Reacts with Shigella species including sonnei. Antiserum is not absorbed and does react with related Enterobacteriaceae.

Characteristics: Rabbit anti Shigella sp., Rabbit antibody to Shigella species

Purification: IgG fraction covalently coupled with the N-Hydroxysuccinimide ester of Biotin under mild conditions to give a high degree of substitution.

Immunogen: Mixture of S. boydii (ATCC 8700), S. flexneri (ATCC 29903) and S. dysenteriae (ATCC 13313)

Application Details

Application Notes: Suitable for use in IFA. Acetone fixation of the antigen source is recommended prior to staining. Spot suspension of bacteria onto slide. Air dry. Fix slide with 100 % Acetone for 5 minutes at room temperature. Air dry. Stain. It is also suitable for conjugation purposes. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M PBS, pH 7.2 Product contains no stabilizing proteins, 0.1 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid multiple freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Short-term (up to 6 months) store at 2-8 °C. Long term, aliquot and store at -28 °C.

Expiry Date: 6 months

Target Details for Shigella

Target: Shigella

Alternative Name: Shigella species

Target Type: Species