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Smith Antigen antibody

The Human Polyclonal anti- antibody has been validated for ID and ELISA. It is suitable to detect in samples from Human.
Catalog No. ABIN238115

Quick Overview for Smith Antigen antibody (ABIN238115)

Target

Smith Antigen

Reactivity

Human

Host

  • 3
Human

Clonality

  • 2
Polyclonal

Conjugate

  • 3
Un-conjugated

Application

  • 2
  • 1
  • 1
Immunodiffusion (ID), ELISA
  • Cross-Reactivity (Details)

    Shows the Sm precipitate band when tested using double immunodiffusion against rabbit thymus extract. Specificity is verified by identity of precipitation with CDC-AF reference serum. Anti-RNP precipitant is also detected. Titer will vary with test system, however, undiluted antibody shows a strong Sm precipitate band. Exhibits no reactivity at a dilution of 1:100 when tested against purified SSB, Scl-70 and Jo-1 antigens in an ELISA system designed to detect all IgG subclasses. Anti-RNP and low levels of anti-SSA activity are detected in this ELISA system.

    Characteristics

    Human anti Sm antigen Human antibody to Sm (Smith) antigen

    Purification

    Single donor human plasma is delipidized and defibrinated. An immunoglobulin fraction is then precipitated and collected. Human plasma
  • Application Notes

    Suitable for use in Immunodiffusion and ELISA. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

    Comment

    Reconstitute with 2ml deionized water.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Buffer

    Lyophilized from 0.01 M Phosphate, 0.11 M Sodium chloride, pH 7.2, 0.02 % Sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid multiple freeze/thaw cycles.
    Centrifuge product if not completely clear after standing at room temperature.
    Prepare working dilution only prior to immediate use.

    Storage

    0 °C

    Storage Comment

    Store at < 0 °C.
  • Target

    Smith Antigen

    Alternative Name

    Sm (Smith) Antigen
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