Orf1 antibody
The Rabbit Polyclonal anti-not_set antibody has been validated for WB. It is suitable to detect not_set in samples from Neisseria meningitidis.
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Catalog No. ABIN238346
$528.00
Plus shipping costs $50.00
0.1 mg
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United States
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Quick Overview for Orf1 antibody (ABIN238346)
Target
Orf1
Reactivity
Neisseria meningitidis
Host
Rabbit
Clonality
Polyclonal
Conjugate
Un-conjugated
Application
Western Blotting (WB)
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Purification
- Protein-A affinity chromatography.
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Immunogen
- Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.
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Application Notes
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Suitable for Western Blotting under Reducing conditions (0.2-1 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- PBS, pH 7.4 with 15 mM sodium azide as preservative.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Orf1
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Target Type
- Viral Protein
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Background
- Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.
Target
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