CD163 antibody

Catalog No. ABIN238373

The Mouse Monoclonal anti-CD163 antibody has been validated for WB, FACS, IHC (p), IHC (fro) and EIA. It is suitable to detect CD163 in samples from Human.

Quick Overview for CD163 antibody (ABIN238373)

Target: CD163

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD163 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 5C6-FAT

Product Details anti-CD163 Antibody

Specificity: Monoclonal antibody 5C6 FAT recognizes a membrane glycoprotein on monocytes and macrophages which is expressed in intermediate and late inflammatory stages. Antigen distribution: Isolated cells: Monocytes, particularly after dexamethasone treatment or after 2-5 days in culture. Does not react with lymphocytes, granulocytes or platelets. Tissue Sections: Positive staining can be observed in the skin (histiocytes), gut, Kupffer cells, few alveolar macrophages, a major population of macrophages in the placenta, varying degrees of macrophages in inflamed tissues, including tumorous tissue depending on the inflammatory stage. Red pulp, but not white pulp macrophages of the spleen, and cortical macrophages of the thymus are detected. Macrophages in the synovialis of patients with rheumatoid arthritis. In alveolar macrophages and in Kupffer cells a double staining can be observed with monoclonal antibody 25F9 (product BM4022) which is not the case in other tissues.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography.

Immunogen: Human CD163. The epitope has not been further characterized.

Isotype: IgG1

Application Details

Application Notes: ELISA. Immunohistochemistry on Frozen Sections: 0.5 μg/mL (1/4000)Immunohistochemistry on Paraffin Sections: 1 μg/mL (1/2000) (Proteinase K pretreatmentfor antigen retrieval is recommended). Suggested Positive Control: Human placenta. Has been described to work in FACS and Western blots.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 2.0 mg/mL

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one month. Aliquot and store at -20 °C for longer.

Target Details for CD163

Target: CD163

Alternative Name: CD163

Background: CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163-positive cells include skin histiocytes, Kupffer cells, spleen macrophages of the red pulp, and some thymus macrophages. The antigen is also found abundantly in human term placenta, and regularly in acute and chronic inflammatory lesions.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130

Gene ID: 9332

NCBI Accession: NP_004235

UniProt: Q86VB7