Interferon gamma antibody (AA 22-166)

Catalog No. ABIN238405

Product Details anti-Interferon gamma Antibody

Target: Interferon gamma (IFNG)

Binding Specificity: AA 22-166

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Specificity: The antibody G-23 reacts with IFN-gamma, a 20-25 kDa cytokine produced by activated Th1 cells and NK cells.

Characteristics: Synonyms: IFN-gamma, gamma IFN

Purification: Affinity Chromatography on Protein A.

Immunogen: Recombinant human IFN-gamma (aa 22-166 representing mature IFN-gamma).

Clone: G-23

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry (2-4 μg/mL)Positive Control: PMA/ionomycin stimulated Peripheral Blood Lymphocytes (PBL)Application note: Intracellular stainingWestern Blotting (1 μg/mL)Positive control: recombinant human IFN-gamma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4 containing 15 mM Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG / Interferon gamma (IFNG Products)

Background: The Interferon gamma (IFN-gamma, 20-25 kDa) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-alpha and IL-12, its production is suppressed by IL-4, IL-10, and TGF-beta. The producing of IFN-gamma is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers, 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-gamma belongs to the type II interferons, also called immune IFN. IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells.Synonyms: IFN-gamma, gamma IFN

Gene ID: 3458

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy