The antibody HCG-61 reacts with beta-subunit of human chorionic gonadotropin, a 40 kDa hormone. hCG belongs to the glycoprotein hormone family together with lutropin (LH), follitropin (FSH) and thyrotropin (TSH). hCG is synthesised by trophoblastic cells of the placenta during pregnancy and stimulates the growth of corpus luteum. The HCG-61 antibody reacts with association constant 5.1 x 10e10 l/mol. Following cross-reactivity (%) was determined by classic double-antibody RIA using unlabelled hormones: beta-hCG (77), alpha-hCG (1.3), hLH (0.86), hFSH (< 0.5), hTSH (< 0.5).
Suitable for Immunocytochemistry and ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.4 15 mM sodium azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date
12 months
Target
hCG
(Chorionic Gonadotropin (hCG))
Target Type
Hormone
Background
HCG is a protein of the molecular weight about 40 kDa. hCG belongs to the glycoprotein hormone family together with lutropin (LH), follitropin (FSH) and thyrotropin (TSH). hCG is synthesised by trophoblastic cells of the placenta during pregnancy and stimulates the growth of corpus luteum. The other hormones are produced by anterior pituitary gland. All these hormones are structurally related being composed of two noncovalently associated subunits α and β.Synonyms: CGB, CGB3, Choriogonadotropin subunit beta, beta hCG, hCG-beta