Suitable for Flow Cytometry analysis of human blood cells using 20 m l reagent / 100 m l of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
For Research Use only
PBS containing 15 mM Sodium Azide as preservative and 0.2 % (w/v) high-grade BSA (Protease free) as stabilizer
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Store the antibody in the dark at 2-8 °C. Do Not Freeze! Avoid prolonged exposure to light. Shelf Life: one year from despatch.
CD29 (beta1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 dibberent alpha chains (alpha1-alpha6, CD49a-f) determining the binding properties of beta1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most beta1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of beta1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a beta1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta.Synonyms: FNRB, Fibronectin receptor subunit beta, ITGB1, Integrin VLA-4 subunit beta, MDF2, MSK12