INPP5D antibody (N-Term)

Catalog No. ABIN238500

This anti-INPP5D antibody is a Mouse Monoclonal antibody detecting INPP5D in WB and EIA. Suitable for Human.

Quick Overview for INPP5D antibody (N-Term) (ABIN238500)

Target: INPP5D (Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This INPP5D antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: SHIP-02

Product Details anti-INPP5D Antibody

Binding Specificity: N-Term

Specificity: The antibody SHIP-02 reacts with SHIP-1, a phosphoinositide phosphatase largely confined to hematopoietic cells. Multiple forms of SHIP-1 have been reported with molecular weights of 110, 125, 130, 135 and 145 kDa.

Characteristics: Synonyms: SHIP, SHIP1, SHIP-1, p150Ship, SIP-145, Phosphatidylinositol-3, 4, 5-trisphosphate5-phosphatase 1, SH2 domain-containing inositol-5'-phosphatase 1, Inositolpolyphosphate-5-phosphatase of 145 kDa

Purification: Protein A affinity chromatography.

Immunogen: Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1.

Isotype: IgG2a

Application Details

Application Notes: Western Blotting (Reducing conditions)Positive control: RAMOS human cell line. Sample preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith reducing Laemmli SDS-PAGE sample buffer. Boil for 6 min. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for INPP5D

Target: INPP5D (Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D))

Alternative Name: INPP5D

Background: SHIP-1 (SH2 domain containing inositol phosphatase-1) is a 5´inositol phosphatase that regulates cell responses in lymphocytes and myeloid cells by hydrolyzing the second messenger PI(3,4,5) trisphosphate. SHIP-1 is recruited upon engagement of both inhibitory and activatory receptors, such as FcgammaRIIB, Fcgamma RIII, FcepsilonRI or cytokine and growth factor receptors, and supresses PI3K-dependent signaling, down-regulates cell migration and invasion of transformed cells and phagocytosis. SHIP-1 also serves as a scaffold for the recruitment of other proteins to the plasma membrane.Synonyms: 4, 5-trisphosphate 5-phosphatase 1, Inositol polyphosphate-5-phosphatase of 145 kDa, Phosphatidylinositol-3, SH2 domain-containing inositol-5'-phosphatase 1, SHIP, SHIP-1, SHIP1, SIP-145, p150Ship

Gene ID: 3635

UniProt: Q92835

Pathways: TCR Signaling, BCR Signaling, Warburg Effect