TUBG1 antibody (C-Term)

Catalog No. ABIN238522

This Mouse Monoclonal antibody specifically detects TUBG1 in WB and IF. It exhibits reactivity toward Human, Rat, Mouse, Chicken, Plant, Protozoa and Pig.

Quick Overview for TUBG1 antibody (C-Term) (ABIN238522)

Target: TUBG1 (Tubulin, gamma 1 (TUBG1))

Reactivity: Human, Rat, Mouse, Chicken, Plant, Protozoa, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: TU-30

Product Details anti-TUBG1 Antibody

Binding Specificity: AA 434-449, C-Term

Specificity: The antibody TU-30 recognizes C-terminal peptide sequence of gamma-Tubulin.

Characteristics: Synonyms: Tubulin gamma-1 chain, Gamma-1-tubulin, GCP-1, Gamma-tubulin complex component 1

Purification: Protein-A Affinity Chromatography

Immunogen: C-terminal peptide (aa 434-449) of gamma-tubulin counjugated to KLHAA Sequence: EYHAATRPDYISWGTQ

Isotype: IgG1

Application Details

Application Notes: Immunocytochemistry (Purified Antibody: 1-2 μg/mL, Staining technique: (a) Fix cells for 10 min in methanol at -20C and for 6 min in acetone at -20C, (b) Fix cells directly inmethanol for 10 min at -20C or in acetone for 10 min at -20C. Incubation: 45 min in roomtemperature, Positive control: P-19 mouse embryonal carcinoma cell line 3T3 mousefibroblasts, Application note: The antibody TU-30 stains only fixed cells). Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4 with 15 mM Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for TUBG1

Target: TUBG1 (Tubulin, gamma 1 (TUBG1))

Alternative Name: TUBG1 / Tubulin gamma 1

Background: The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01 % of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.Synonyms: GCP-1, Gamma-1-tubulin, Gamma-tubulin complex component 1, Tubulin gamma-1 chain

Gene ID: 7283

UniProt: P23258

Pathways: Microtubule Dynamics, M Phase