RPL22 antibody (C-Term)

Catalog No. ABIN238580

The Goat Polyclonal anti-RPL22 antibody has been validated for IHC and ELISA. It is suitable to detect RPL22 in samples from Human.

Quick Overview for RPL22 antibody (C-Term) (ABIN238580)

Target: RPL22 (Ribosomal Protein L22 (RPL22))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RPL22 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-RPL22 Antibody

Binding Specificity: C-Term

Purpose: Ribosomal protein L22

Sequence: SKESYELRYF QINQ

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SKESYELRYFQINQ, from the C Terminus of the protein sequence according to NP_000974.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Brain (Cortex) shows cytoplasm staining in neuronal bodies. Recommended concentration: 3-6 μg/mL. Paraffin embedded Human Thyroid, Uterus and Testis. Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary experiments gave bands at approx 17-18 kDa and 25 kDa in Human Liver lysates after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculate

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RPL22

Target: RPL22 (Ribosomal Protein L22 (RPL22))

Alternative Name: RPL22

Background: RPL22, ribosomal protein L22, EAP, HBP15, HBP15/L22, 60S ribosomal protein L22, EBER-associated protein, Epstein-Barr virus small RNA-associated protein, Epstein-Barr-encoded RNA-associated protein, heparin-binding protein 15, heparin-binding protein HBp1

Gene ID: 6146, 19934, 81768

NCBI Accession: NP_000974