ABCB5 antibody (Internal Region)

Catalog No. ABIN238622

The Goat Polyclonal anti-ABCB5 antibody has been validated for WB, ELISA and FACS. It is suitable to detect ABCB5 in samples from Human. There is 1 publication available.

Quick Overview for ABCB5 antibody (Internal Region) (ABIN238622)

Target: ABCB5 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ABCB5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-ABCB5 Antibody

Binding Specificity: Internal Region

Purpose: ABCB5

Sequence: QTQHRNTSKK AQ

Specificity: This antibody is expected to recognise both reported isoforms (NP_848654.3, NP_001157413.1)

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QTQHRNTSKKAQ , from the internal region of the protein sequence according to NP_848654.3, NP_001157413.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 80 kDa band observed in lysates of cell line A431(calculated MW of 89.8 kDa according to NP_848654.3). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-ABCB5 antibody (ABIN238622)

Cheung, Cheung, Cheng, Wong, Fan: "Granulin-epithelin precursor and ATP-dependent binding cassette (ABC)B5 regulate liver cancer cell chemoresistance." in: Gastroenterology, Vol. 140, Issue 1, pp. 344-55, (2010) (PubMed).

Target Details for ABCB5

Target: ABCB5 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5))

Alternative Name: ABCB5

Background: ATP-binding cassette, sub-family B (MDR/TAP), member 5 , ABCB5alpha, ABCB5beta, EST422562, ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5

Molecular Weight: 89.8kDa

Gene ID: 340273

NCBI Accession: NP_848654, NP_001157413